Electrophoretic Heterogeneity of Polypeptide Chains of Specific Antibodies

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Science  27 May 1966:
Vol. 152, Issue 3726, pp. 1253-1254
DOI: 10.1126/science.152.3726.1253


Heavy and light polypeptide chains isolated from different specific antibodies to haptens and from γG-immunoglobulin of normal rabbits have been resolved into distinct, multiple components by disc electrophoresis in polyacrylamide gels in the presence of urea. In spite of the resolution of these chains into multiple bands, different specific antibodies and normal rabbit γG-immunoglobulin were indistinguishable from each other by this method.