Transformation of Auxotrophic Mutants of Group H Streptococci

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Science  27 May 1966:
Vol. 152, Issue 3726, pp. 1255-1256
DOI: 10.1126/science.152.3726.1255


Stable auxotrophic mutants of a group H streptococcus (strain Challis) were isolated on a modified Mickelson defined medium after exposure to N-methyl- Ń-nitro-N-nitrosoguanidine. Such mutants were transformed to both prototrophy and streptomycin-resistance and hence may be used as nutritional markers in the study of streptococcal genetics.