Versatile Perfusion Chamber for Living Cells and Organs

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Science  24 Jun 1966:
Vol. 152, Issue 3730, pp. 1758-1760
DOI: 10.1126/science.152.3730.1758


A microperfusion chamnber, which provides controlled circulation of nutrient with chamber depths varying between 15 and 1500 microns and which permits the maintenance of transverse concentration gradients of nutrient constituents or gases, is described. The added advantage of facilitating controlled flow between adjacent chambers on a standard slide (38 x 76 mm) makes the chamber useful in the study of both plant and animal materials. Examples of such use are given.