Enzyme-Coenzyme Complexes of Pyridine Nucleotide-Linked Dehydrogenases

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Science  22 Jul 1966:
Vol. 153, Issue 3734, pp. 414-415
DOI: 10.1126/science.153.3734.414


Enzyme-reduced coenzyme binary complexes produce previously unreported shifts in the spectrum of the free coenzyme. These shifts give rise to difference spectra which resemble a general environmental change for reduced diphosphopyridine nucleotide (DPNH) in the glutamic dehydrogenase-DPNH complex, and indicate a more specific enzyme-coenzyme interaction for yeast alcohol dehydrogenase-DPNH, isocitrate dehydrogenase-TPNH, and lactic dehydrogenase-DPNH complexes.