Biosynthesis of Gamma Globulin: Studies in a Cell-Free System

See allHide authors and affiliations

Science  29 Jul 1966:
Vol. 153, Issue 3735, pp. 537-539
DOI: 10.1126/science.153.3735.537


Incorporation of C14-amino acids into high-molecular-weight material precipitable by trichloroacetic acid indicates that microsomal cell-free systems, derived from spleens of immunized rabbits, are active in protein synthesis. Protein was made soluble by ultrasonic irradiation of the cell-free incubation mixtures, and low-molecular-weight materials were removed by dialysis and gel filtration. Chromatography and radio-immunoelectrophoresis of this soluble protein fraction reveal C14-labeled protein having several characteristics of gamma globulin.