Selection of Sucrose-Dependent Escherichia coli To Obtain Envelope Mutants and Fragile Cultures

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Science  19 Aug 1966:
Vol. 153, Issue 3738, pp. 892-894
DOI: 10.1126/science.153.3738.892


Mutants isolated as sucrose-dependent include many with apparent envelope defects, which fact often leads to filamentous growth or lysis in the absence of sucrose. One mutant can grow exponentially in 4 percent sucrose, but is very fragile: it releases all of its RNA and soluble protein when treated with 0.5 percent sodium deoxycholate. These characteristics permit the study of unstable structures and rapid processes in actively growing cells.