Immunization of Normal Mouse Spleen Cell Suspensions in vitro

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Science  26 Aug 1966:
Vol. 153, Issue 3739, pp. 1004-1006
DOI: 10.1126/science.153.3739.1004

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Dissociated cells from the spleens of unimmunized mice were cultured with and without various mammalian erythrocytes. Spleen cell suspensions cultured with heterologous red cells developed levels of hemolytic plaque-forming cells only one log2 less than those seen in vivo. The reaction is specific for the in vitro immunizing erythrocytes. Antibody was demonstrated in the culture fluids.