Differentiation in vitro: Effects of Sephadex Fractions of Chick Embryo Extract

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Science  02 Sep 1966:
Vol. 153, Issue 3740, pp. 1116-1119
DOI: 10.1126/science.153.3740.1116


Chicken embryo extract has been fractionated into high-and low-molecular-weight components on Sephadex G-25. Media supplemented with the low-molecular-weight fraction (L) support full differentiation in clones of cartilage and of pigmented retina cells from chicken embryos. Growth rates of such cultures in L-supplemented media are greater than in media without embryo extract, and plating efficiencies are higher than in media with or without whole embryo extract. The high-molecular-weight fraction (H) in low concentrations also stimulates growth and plating efficiency, but inhibits the expression of differentiation.