Nucleic Acid Guanine: Reaction with the Carcinogen N-Acetoxy-2-Acetylaminofluorene

See allHide authors and affiliations

Science  02 Sep 1966:
Vol. 153, Issue 3740, pp. 1125-1127
DOI: 10.1126/science.153.3740.1125


Reaction of N-acetoxy-2-acetylaminofluorene with DNA or RNA at pH 7 causes marked increase in absorption at 280 to 320 millimicrons and marked decrease in guanine content. Reaction with guanosine-8-14C yields a radioactive fluorescent derivative. The data suggest that metabolic esters of N-hydroxy-2-acetylaminofluorene may be intermediates in the binding of this proximate carcinogen to nucleic acids in vivo.