Electrochromatography with Reversing Electrophoretic Field

Science  16 Sep 1966:
Vol. 153, Issue 3742, pp. 1381-1382
DOI: 10.1126/science.153.3742.1381


A protein mixture can be separated in a flow column by application of a transverse electrophoretic field to carry faster-migrating components of the mixture into a fixed gel bed at one side of the column, so that slower components move along in a buffer stream. The field is then reversed to returnthe faster-moving components to the buffer stream. The cycle is repeated many times to complete the separation.