Cilia Isolated from Tetrahymena after Membrane Stabilization by 1,5-Difluoro-2,4-Dinitrobenzene

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Science  16 Sep 1966:
Vol. 153, Issue 3742, pp. 1390-1391
DOI: 10.1126/science.153.3742.1390


The tendency for Tetrahymena pyriformis cells to lyse when they are centrifuged at speeds above 120g and then exposed to an ethanolic deciliating medium is inhibited by treatment with 1,5-difluoro-2,4-dinitrobenzene. This treatment facilitates greater yields and purer preparations of isolated cilia. Cilia from treated and untreated cells do not differ in some of their biochemical properties.