Coding Properties of 7-Methylguanine

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Science  16 Sep 1966:
Vol. 153, Issue 3742, pp. 1403-1405
DOI: 10.1126/science.153.3742.1403


The abnormal base, 7-methylguanine, has been introduced into copolymers of uridylic and guanylic acids by methylation under controlled conditions. The resulting methylated copolymers have a markedly decreased template activity for polypeptide synthesis in vitro due to steric effects of the 7-methyl groups. Contrary to expectation, these polymers do not permit incorporation of amino acids whose codons contain the bases uracil and adenine.