Purification and Reconstitution of the Periodic Fibril and Unit Structure of Human Amyloid

Science  14 Oct 1966:
Vol. 154, Issue 3746, pp. 271-272
DOI: 10.1126/science.154.3746.271


Extraction with alkaline sodium glycinate of the periodic fibril and unit structure of human amyloid and subsequent acidification of the extract provided a method for the purification as well as for the reconstitution of the periodic rod and of the unit structure. Guanidine completely solubilized and irreversibly dissociated both the rod and the unit structure of the human amyloid fibril.