Protoplasts: Preparation from Higher Plants

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Science  14 Oct 1966:
Vol. 154, Issue 3746, pp. 280-281
DOI: 10.1126/science.154.3746.280


Protoplasts can be readily prepared from higher-plant tissue with a concentrated Myrothecium cellulase preparation. An ionic osmoticum produced a higher yield and more viable protoplasts than did the nonionic man nitol osmoticum. Addition of polyuronidases or of ethylenediaminetetra-acetate did not increase the yield. Protoplasts could be prepared from root, leaf, and callus tissue as well as from coleoptiles.