Gamma-A Cold Agglutinin: Importance of Disulfide Bonds in Activity and Structure

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Science  14 Oct 1966:
Vol. 154, Issue 3746, pp. 281-283
DOI: 10.1126/science.154.3746.281


Cleavage of disulfide bonds caused by mild reduction with mercaptoethanol produces a reversible loss of cold agglutinin activity and antigen-binding ability in a gamma-A polymer cold agglutinin. Return of agglutination and antigen-binding ability occur only in those molecules that have not been depolyimerized by reduction. Loss of activity becomes irreversible if reduction is followed immediately by alkylation.