Protein- Polysaccharide Loss during Endochondral Ossification: Immunochemical Evidence

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Science  21 Oct 1966:
Vol. 154, Issue 3747, pp. 393-395
DOI: 10.1126/science.154.3747.393


Fluorescein labeled antibodies to protein- polysaccharides from rat and calf cartilage were used for the histochemical localization of protein- poly saccharide in epiphyseal cartilage. There was less protein- polysaccharide in the zone of provisional calcification than in the proliferating and maturing zones, and none was demonstrable in the metaphysis. During or just preceding cal cification, protein- polysaccharide or its protein component is lost or drastically altered.