Reaggregation of Insect Cells as Studied by a New Method of Tissue and Organ Culture

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Science  28 Oct 1966:
Vol. 154, Issue 3748, pp. 516-517
DOI: 10.1126/science.154.3748.516


Cells of the dissociated pupal fat of saturniid moths reaggregated when maintained in vitro in a new type of chamber that permits the culture to "respire" through a film of polyethylene. The fat-body cells are mutually cohesive, but depend on ameboid hemocytes to bring them together. The hemocytes are also capable of causing the reaggregation of particles of diethylaminoethyl-Sephadex.