Allomorphic Forms of Bacteriophage øX-174 Replicative DNA

Science  04 Nov 1966:
Vol. 154, Issue 3749, pp. 658-660
DOI: 10.1126/science.154.3749.658


The bacteriophage øX-174 replicative form of DNA exists in two configurations, as judged by electron microscopy and sucrose density gradient sedimentation, and on methylated albumin-coated kieselguhr column chromatography.The predominant 21S form of the DNA is a tightly twisted circle. Treatment with deoxyribonuclease causes a single scission in one of the two strands, resulting in an open circle which sediments at 16S.