Enhanced Growth of Human Embryonic Cells Infected with Adenovirus 12

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Science  04 Nov 1966:
Vol. 154, Issue 3749, pp. 665-667
DOI: 10.1126/science.154.3749.665


Fibroblast-like cells from human embryonic lung were infected with adenovirus type 12, and they survived as an established line, with the characteristics of "transformation" following considerable cellular killing. Inclusion bodies disappeared and cells became resistant to reinfection with type 12 virus as they grew in thick multilayered strands, and giant and syncytial cells became commonplace. An induced new cell antigen demonstrable by complement-fixation and fluorescent-antibody studies persisted for at least 20 culture passages after infection.