Co-linearity of β-Galactosidase with Its Gene by Immunological Detection of Incomplete Polypeptide Chains

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Science  25 Nov 1966:
Vol. 154, Issue 3752, pp. 1027-1029
DOI: 10.1126/science.154.3752.1027


Many nonsense mutants that map in the β-galactosidase structural gene produce material that forms precipitin lines when tested by double diffusion on agar against antiserum prepared from native β-galactosidase. Relative sizes of the cross-reacting materia!s measured by sucrose density gradient centrifugation are the same as sizes calculated from genetic mapping of nonsense mutants. Orientation of the protein to its gene is also indicated.