Reports

Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield

+ See all authors and affiliations

Science  30 Dec 1966:
Vol. 154, Issue 3757, pp. 1662-1665
DOI: 10.1126/science.154.3757.1662

Abstract

A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver. It is a modification of methods that use concentrated sucrose solutions rather than citric acid or detergents and, therefore, permits further quantitative fractionation of cytoplasmic components.