Acetylcholinesterase: Method for Demonstration in Amacrine Cells of Rabbit Retina

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Science  27 Jan 1967:
Vol. 155, Issue 3761, pp. 477-478
DOI: 10.1126/science.155.3761.477


The activity of acetylcholinesterase in the inner plexiform layer of the rabbit retina was not affected detectably by prior section of the optic nerve. After the animals were treated with diisopropyl phosphorofluoridate, acetylcholinesterase reappeared in the somata of the amacrine cells and in certain cells of the ganglion cell layer before it reappeared in the inner plexiform fibers. This confirms the normal presence of acetylcholinesterase at the former site. The possible role of acetylcholine in intraretinal transmission is considered.