Sporulation Mutations Induced by Heat in Bacillus subtilis

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Science  17 Feb 1967:
Vol. 155, Issue 3764, pp. 838-839
DOI: 10.1126/science.155.3764.838


When spores of Bacillus subtilis strain Marburg are heated (90° to 100°C) in a vacuum for 9 to 12 hours and then plated, numerous mutants are obtained, and very few spores are killed. Disproportionately large numbers of these mutants exhibit abnormal sporulation.