Artifact Produced in Disc Electrophoresis by Ammonium Persulfate

Science  14 Apr 1967:
Vol. 156, Issue 3772, pp. 256-257
DOI: 10.1126/science.156.3772.256


Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.