Fluorescence Decay Times: Proteins, Coenzymes, and Other Compounds in Water

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Science  19 May 1967:
Vol. 156, Issue 3777, pp. 949-951
DOI: 10.1126/science.156.3777.949


The fluorescence decay time (τ) was 2 to 5 nanoseconds for proteins and 4 to 5 nanoseconds for flavin, pyridine nucleotide, and vitamin B6coenzymes; τ varied widely in 48 compounds measured in water. Altholugh reported values of τ for a few of the soluttions studied were in excellenlt agreement. previously "calculated" lifetimes, in severall instances, are apparently erroneous. Nonexponential decay was detectable with our "nanosecond-flash" apparatus, a modification of the first commercially aavilable unit for determination of τ.