Symbiosis: Effects of a Mutualistic Fungus upon the Growth and Reproduction of Xyleborus ferrugineus

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Science  26 May 1967:
Vol. 156, Issue 3778, pp. 1120-1122
DOI: 10.1126/science.156.3778.1120


Xyleborus ferrugineus beetles developed from asymbiontic eggs through the adult stage on a sterilized meridic diet, but the resulting adults reproduced only when a mutualistic fungus was inoculated into the diet. Beetles with bacterial symbionts still required the fungus for reproduction on a second meridic diet.