Dimethyl Sulfoxide Protects Tightly Coupled Mitochondria from Freezing Damage

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Science  30 Jun 1967:
Vol. 156, Issue 3783, pp. 1738-1739
DOI: 10.1126/science.156.3783.1738


Dimethyl sulfoxide prevented loss of respiratory control and decrease in efficiency of oxidative phosphorylation when plant mitochondria were stored in liquid nitrogen. Respiration was severely inhibited and was not stimulated by adenosine diphosphate when mitochondria were frozen in liquid nitrogen without dimethyl sulfoxide. Thus, isolated mitochondria provide a model system for the study of the effects of freezing on biological membranes and of the prevention, by dimethyl sulfoxide, of freezing damage.