Light Chains of Rabbit Immunoglobulin: Assignment to the Kappa Class

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Science  30 Jun 1967:
Vol. 156, Issue 3783, pp. 1755-1757
DOI: 10.1126/science.156.3783.1755


Normal rabbit gamma globulin was reduced under conditions presumed to break only interchain disulfide bridges, and the reduiced product was then blocked with C14-iodoacetamide. The light chains were separated from the heavy chains and subjected to peptic digestion. Two radioactive peptides were recovered from the digest. The peptides are apparently overlapping and represent the carboxyterminuis. Comparison of this region in the rabbit light chains with the corresponding amino acid sequences in various mouse and human light chains indicates that the rabbit light chains are of the K-class.