Genetic Mapping of Phenylalanyl-sRNA Synthetase in Escherichia coli

See allHide authors and affiliations

Science  07 Jul 1967:
Vol. 157, Issue 3784, pp. 78-79
DOI: 10.1126/science.157.3784.78


Genetic mapping of the structural gene for a phenylalanyl-sRNA synthetase in Escherichia coli was accomplished by the use of mutants with temperature-sensitive or p-fluorophenylalanine-resistant enzymes. The structural gene is located at minute 33 of the Taylor-Thoman map of the E. coli chromosome, closely linked to a structural gene (aroD) for one of the enzymes involved in the biosynthesis of the aromatic amino acids, and distant from the known locations of other aminoacyl-sRNA synthetase genes.