Replication of Viral RNA: RNA Synthetase from Escherichia coli Infected with Phage MS2 or Qβ

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Science  11 Aug 1967:
Vol. 157, Issue 3789, pp. 701-703
DOI: 10.1126/science.157.3789.701


An RNA synthetase is formed in Escherichia coli after infection with bacteriophages containing RNA. Specific annealing techniques revealed that, from the very outset of the reaction in vitro, the partially purified enzyme-template complex synthesizes parental-type plus strands, namely, MS2-RNA when isolated from cells infected with MS2 phage and Qβ-RNA in the case of cells infected with Qβ phage. This is in contrast to the situation found with Qβ replicase primed with Qβ-RNA, where the initial product is the complementary strand.