Electron Spin Resonance of Gamma-Irradiated Oriented DNA Prepared by Wet Spinning

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Science  15 Sep 1967:
Vol. 157, Issue 3794, pp. 1317-1319
DOI: 10.1126/science.157.3794.1317


When moist, oriented DNA is gamma-irradiated and its electron spin resonance spectrum is recorded at 77°K, an unresolved spectrum is obtained, the amplitude of which is strongly dependent on the angle between the direction of the DNA helices and the field. Annealing at 199°K gives an eight-line thymine-like spectrum which also has a marked angular dependence. For dry, oriented DNA, the unresolved spectrum dominates even at room temperature, and the spectra exhibit lower degrees of anisotropy.