Separation of Spores from Diploid Cells of Yeast by Stable-Flow Free-Boundary Electrophoresis

Science  10 Nov 1967:
Vol. 158, Issue 3802, pp. 803-804
DOI: 10.1126/science.158.3802.803


By the use of stable-flow free-boundary (Staflo) electrophoresis and the electrophoretic mobility difference between ascospores and diploid cells of Saccharomyces cerevisiae, a mixture of the two can be separated into spore and diploid cell fractions. The spore fraction that is obtained can then be used for genetic analysis.