Colostral Immunoglobulin-A: Synthesis in vitro of T-Chain by Rabbit Mammary Gland

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Science  17 Nov 1967:
Vol. 158, Issue 3803, pp. 932-933
DOI: 10.1126/science.158.3803.932


When minced mammary tissue from lactating rabbits was incubated in vitro with C14-labeled lysine and isoleucine, it incorporated radio-activity into colostral immunoglobulin A. The only portion of this colostral molecule with significant labeling was T-chain, with little or no labeling of light or heavy chains. It was thus demonstrated that T-chains are synthesized by mammary gland. Because the remainder of the molecule was derived from unlabeled material, in vivo it was probably derived from serum.