A Requirement for Two Cell Types for Antibody Formation in vitro

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Science  22 Dec 1967:
Vol. 158, Issue 3808, pp. 1573-1575
DOI: 10.1126/science.158.3808.1573


A suspension of mouse spleen cells can be separated into two populations on the basis of their ability or inability to adhere to plastic dishes. It was found that both adherent and nonadherent cells were necessary for the induction of antibody formation to sheep red blood cells in vitro. Exposure of adherent cells to antigen for brief periods of time was sufficient to initiate a maximal in vitro response.