Growth of Isolated Mesophyll Cells of Arachis hypogaea in Simple Defined Medium in vitro

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Science  22 Dec 1967:
Vol. 158, Issue 3808, pp. 1575-1577
DOI: 10.1126/science.158.3808.1575


Isolated mesophyll cells from leaflets of Arachis hypogaea can be cultured in a simple, defined liquid nutrient medium containing minerals, with an appropriate source of ammonia, sugar, 2,4-dichlorophenoxyacetic acid, and kinetin. The significance of such a simple medium in understanding the problems of cell metabolism, growth, and morphogenesis is discussed.