Induction and Survival of Hemoglobin-Less and Erythrocyte-Less Tadpoles and Young Bullfrogs

Science  05 Jan 1968:
Vol. 159, Issue 3810, pp. 101-103
DOI: 10.1126/science.159.3810.101


Injection of two 25-microgram-per-gram doses of the hemolytic agent phenylhydrazine reduced the hemoglobin level and the erythrocyte count to less than 1 percent of normal tadpole and young blullfrog blood. These anemic animals survive for weeks with little change in overall metabolism. A slow recovery of hemoglobin levels was observed. The implications of this observation for comparative biochemistry are considered.