Antigen Binding to Cells: Determination by Enzymic Fluorogenic Group Hydrolysis

Science  23 Feb 1968:
Vol. 159, Issue 3817, pp. 884-885
DOI: 10.1126/science.159.3817.884


A sensitive method for detecting cells containing antibody to β-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.