Murine Hepatitis Virus: Effect on Liver RNA

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Science  01 Mar 1968:
Vol. 159, Issue 3818, pp. 987-990
DOI: 10.1126/science.159.3818.987


After infection of mice with hepatitis virus MHV3, the RNA in the liver undergoes changes. The fraction extracted with phenol at 0°C does not alter. However, the fraction extracted with hot phenol at elevated pH (60°C, pH 8.3) shows a 16S peak on sucrose-density-gradient centrifugation. This fraction shows actinomycin D-resistant incorporation of C14-orotic acid in infected but not in control livers—possible evidence of the RNA nature of MHV3.