Mitochondrial Malate Dehydrogenase: Reversible Denaturation Studies

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Science  01 Mar 1968:
Vol. 159, Issue 3818, pp. 997-999
DOI: 10.1126/science.159.3818.997


The malate dehydrogenase isoenzymes from the mitochondria of chicken hearts have been partially resolved into separate pools. Reversible denaturation, in concentrated guanidine hydrochloride, does not change the isoenzyme distribution in each pool. This result suggests that the electrophoretic differences among the isoenzymes are not solely conformational in origin.