Protein Synthesis in the Hippocampal Pyramidal Cells of Rats during a Behavioral Test

Science  22 Mar 1968:
Vol. 159, Issue 3821, pp. 1370-1373
DOI: 10.1126/science.159.3821.1370


Protein synthesis was studied in the pyramidal nerve cells of the CA3 region in the hippocampus of rats uring a behavioral test involving transfer of handedness. A new electrophoretic technique was used for separation of 10-7 to 10-9 gram of protein and radiometric determination of the various protein fractions. After interventricular administration of 3H-leucine, protein synthesis of two fast-moving fractions was significantly higher bilaterally in the hippocampus of the trained rats. There was also a trend to lateralization of the highest protein synthesis to the learning side.