Starch Accumulation in Shoot-Forming Tobacco Callus Cultures

Science  26 Apr 1968:
Vol. 160, Issue 3826, pp. 421-422
DOI: 10.1126/science.160.3826.421


Microscopic histochemical examinations of cultured tobacco callus disclosed a strong correlation between starch accumulation and shoot initiation. The accumulation started before any observable organized development and was heaviest in cells of loci which ultimately gave rise to organ primordia. Treatment of tissue cultures with gibberellin prevented starch accunmulation and organ formation.