Reports

Comparison of RNA Terminal Sequences of Phages f2 and Qβ: Chemical and Sedimentation Equilibrium Studies

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Science  28 Jun 1968:
Vol. 160, Issue 3835, pp. 1459-1460
DOI: 10.1126/science.160.3835.1459

Abstract

The terminal fragment obtained by hydrolysis with ribonuclease T1 of the ribonucleic acid from the bacteriophage Qβ has been isolated and purified. The results of chemical and enzymatic hydrolysis of this fragment and of the intact RNA itself indicate that the fragment has the composition (10 Cp, 4 Up), and that the RNA has the terminal sequence -GP(9 Cp, 4 Up) CpA. These conclusions are supported by the results of an application of the sedimentation-equilibrium method in which the molecular weight of the Qβ fragment was compared with that of the corresponding fragment from f2 phage RNA for which the terminal sequence, -GpUpUpApCpCpApCpCpCpA had previously been determined.