Density Gradient Centrifugation: Fixation of Bands by Photopolymerization of Acrylamide

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Science  26 Jul 1968:
Vol. 161, Issue 3839, pp. 386
DOI: 10.1126/science.161.3839.386


A method for immobilization of proteins in a density gradient is described. This method eliminates the collection of drops or the use of a schlieren optical system, but visualizes the results of the Amido Schwarz stain. Although only sucrose gradients have been examined, the method possibly may be extended to other gradients.