Ruthenium Tetroxide for Fixing and Staining Cytoplasmic Membranes

Science  13 Sep 1968:
Vol. 161, Issue 3846, pp. 1157-1158
DOI: 10.1126/science.161.3846.1157


Rat liver and kidney were fixed for electron microscopy with ruthenium tetroxide. When compared with tissues fixed with osmiun tetroxide, the membranes showed well without additional staining. The trilaminar structuire of the cytoplasmic membranes was clearly seen. In width the nuclear, mitochondrial, and cytoplasmic membranes investigated resembled the cell memnbrane.