Nucleic Acid Molecules: New Microdiffusion Technique for Visualization

Science  20 Sep 1968:
Vol. 161, Issue 3847, pp. 1246-1247
DOI: 10.1126/science.161.3847.1246


A microdiffusion technique, developed for visualization of nucleic acid molecules in the electron microscope, requires less than 0.01 microgram of nucleic acid. Although originally developed for free nucleic acids, the method can be applied to virion suspensions for direct visualization of their genomes; less than 1010 virions per milliliter are required. Results agree well with those yielded by the diffusion technique of Lang, Kleinschmidt, and Zahn.