Sweetclover-Weevil Feeding Deterrent B: Isolation and Identification

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Science  17 Jan 1969:
Vol. 163, Issue 3864, pp. 293-294
DOI: 10.1126/science.163.3864.293


Deterrent B, a compound apparently involved in the resistance of Melilotus infesta to the sweetclover weevil Sitona cylindricollis, has been isolated from leaves by a combination of preparative paper chromatography, sublimation, and crystallization. The compound has been identified as ammonium nitrate. Isolated deterrent B and ammonium nitrate have identical feeding deterrent activities. Although the deterrent principle was isolated as the ammonium salt, nitrate ion is probably responsible for the deterrent activity in vivo.