Cationic Protein-Bearing Granules of Polymorphonuclear Leukocytes: Separation from Enzyme-Rich Granules

Science  07 Mar 1969:
Vol. 163, Issue 3871, pp. 1069-1071
DOI: 10.1126/science.163.3871.1069


The cationic, antibacterial proteins of polymorphonuclear leukocytes are associated with a unique subcellular particle that is separable through zonal density gradient centrifugation from acid phosphatase-containing particles as well as from particles that contain alkaline phosphatase and lysozyme. Normal macrophages, macrophages stimulated by bacillus Calmette-Guérin, and liver cells lack this particle and the associated group of cationic proteins. Particles physically and biochemically similar to slower sedimenting enzyme-rich particles of polymorphonuclear leukocytes are shared by all the tlhree cell types.