Deoxyribonucleic Acid Methylase Activity in Pea Seedlings

Science  09 May 1969:
Vol. 164, Issue 3880, pp. 721-722
DOI: 10.1126/science.164.3880.721


Deoxyribonucleic acid methylase activity has been detected in a preparation of disrupted nuclei prepared from pea seedlings. S-Adenosyl-L-methionine acted as a donor of methyl groups, and the product of the reaction was identified as 5-methylcytosine. The reaction had a sharp temperature optimum at about 30°C and was unusual in that the DNA methylase was able to methylate DNA in the crude extract.