Cell Population Kinetics: A Modified Interpretation of the Graph of Labeled Mitoses

Science  23 May 1969:
Vol. 164, Issue 3882, pp. 952-954
DOI: 10.1126/science.164.3882.952


Graphs of labeled mitoses, derived from autoradiographs of cell populations with 3H-thymidine, show depressions in the curves at their midpoints. These depressions reflect interruption of DNA synthesis midway through S phase. Such interruptions revealed by the method of labeled mitoses should be considered when determining cell-cycle times.