Reports

Human Ooeytes: Maturation in Chemically Defined Media

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Science  13 Jun 1969:
Vol. 164, Issue 3885, pp. 1292-1293
DOI: 10.1126/science.164.3885.1292

Abstract

Human oocytes from ovarian follicles resume meiosis in F10, a defined medium, in numbers comparable to that obtained in medium containing serum. Several simple Krebs-Ringer media also support maturation, which suggests a similarity of nutritional requirements between human and mouse oocytes. Fewer oocytes reach metaphase II when the investing follicular cells are removed prior to culture.